List of matches in this round
- TimeMatch
- January 9, 2021
- 2:15 pmHalifax Vandals RUFC--:--Harrogate Pythons RUFC 1st XV
- January 16, 2021
- 2:15 pmHarrogate Pythons RUFC 1st XV--:--Castleford RUFC
- January 23, 2021
- 2:15 pmBarnsley RUFC--:--Harrogate Pythons RUFC 1st XV
- January 30, 2021
- 2:15 pmHarrogate Pythons RUFC 1st XV--:--Hemsworth RUFC
- February 13, 2021
- 2:15 pmKnottingley RUFC--:--Harrogate Pythons RUFC 1st XV
- February 20, 2021
- 2:15 pmHarrogate Pythons RUFC 1st XV--:--Ossett RUFC
- March 6, 2021
- 3:00 pmSheffield Medicals RUFC--:--Harrogate Pythons RUFC 1st XV
- March 20, 2021
- 3:00 pmHarrogate Pythons RUFC 1st XV--:--Halifax Vandals RUFC
- March 27, 2021
- 3:00 pmCastleford RUFC--:--Harrogate Pythons RUFC 1st XV
- April 10, 2021
- 3:00 pmHarrogate Pythons RUFC 1st XV--:--Barnsley RUFC
- April 17, 2021
- 3:00 pmHemsworth RUFC--:--Harrogate Pythons RUFC 1st XV
- April 24, 2021
- 3:00 pmHarrogate Pythons RUFC 1st XV--:--Knottingley RUFC